Table 5 An example of experimental training at the first stage of the UDBRE system

Cell biology

Isolation and purification of cells and their components; cell culture; use of optical microscope and photomicrography; in situ hybridization; preparation of culture medium;

Molecular biology

Use of Micropipette; western blot; polymerase chain reaction (PCR); agarose gel electrophoresis; extraction of genomic DNA from eukaryotic cells; restriction enzyme digestion;

Oral microbiology

Isolation and culture of bacteria; medium transfer technique; common bacteria identification and staining methods; drug sensitivity test; germicidal test; use of oil immersion objective; preparation and inoculation of culture medium;

Dental materials

Methods for materials component analysis (infrared spectrometry, chromatography, mass spectrometry) and materials surface analysis (use of optical microscopic, scanning electron microscope, atomic force microscope); tests for bonding, curing, and mechanical property, deformation, hardness, and fluidity.

Oral and maxillofacial oncology

Fluorescent quantitative PCR; flow cytometry; immunofluorescence technique; immunohistochemical technique; identification of protein by mass spectrometry;

Oral and maxillofacial histology and pathology

Making paraffin sections; cell staining (HE staining; immunofluorescence staining);