Table 2 Tasks in the TBL model for immunohistochemistry and the task of hematoxylin and eosin staining as an example
Principle tasks | Learning objectives |
|---|---|
Experimental methods | Paraffin section technique; Frozen section technique |
HE staining; Silver staining | |
Nissl staining; Giemsa staining | |
Oil red O dyeing; Hoechst staining | |
Tissue microarrays; In situ hybridization | |
Periodic acid–Schiff (PAS) reaction | |
Immunoperoxidase immunohistochemistry | |
Immunofluorescence histochemistry | |
Experimental tools | Confocal microscopy; Flow cytometer |
Scanning electron microscope (SEM) | |
Transmission electron microscopy (TEM) | |
Immunoelectron microscopy | |
Quantification of morphometric analysis | Quantification of morphological images |
Stereology | |
Questions | |
1. What is your research project? Do you use hematoxylin-eosin staining (HE staining) in your research work? (Phase 1) | |
2. What is the principle of HE staining? (Phase 1) | |
3. What problems HE staining can solve? (Phase 1) | |
4. What reagents are needed in this experiment? How to configure these buffer solutions? (Phase 3) | |
5. What is the experimental protocol of HE staining? (Phase 3) | |
6. How observed the experimental results of HE staining? (Phase 3) | |
7. There are some key options and considerations to take into account.(Teachers provided, Phase 4) | |
8. What are the advantages and disadvantages of HE staining compared with other methods? (Teachers provided, Phase 5) | |